Evaluation of different API systems for identification of porcine Pasteurella multocida isolates

Identification of Pasteurella multocida and Pasteurella haemolytica by API 20E, Minitek, and Oxi/Ferm systems.

Fifty serotyped isolates each of Pasteurella multocida and Pasteurella haemolytica were tested on the API 20E strip (Analytab Products, Plainview, N.Y.), the Oxi/Ferm tube (Roche Diagnostics, Nutley, N.J.), and the Minitek system (BBL Microbiology Systems, Cockeysville, Md.). None of the rapid test systems reliable identified these organisms. With the API system, discrepancies between expected ...

Polypeptide Mapping of Different Isolates of Pasteurella multocida Bovine Origin

The protein analysis of Pasteurella multocida organism was performed by sodium dodycyle sulphate polyacrylamide gel electrophoresis (SDS-PAGE) technique using a high pH discontinous buffer system. Six isolates of the Pasteurella multocida organism were confirmed by Gram’s staining and different biochemical tests. In the protein fractions of purified Pasteurella multocida strains, seven polypept...

Isolation typing of Pasteurella multocida poultry isolates from IRAN

Molecular identification and capsular typing of Pasteurella multocida isolates from sheep pneumonia in Iran

Pasteurella multocida is known as one of the main organisms causing pneumonia in sheep. As immunity in pasteurellosis is serogroup specific, identification of prevalent capsular group among endemic areas is essential. The aim of this study was to molecular identification and determine of the capsular type of the P. multocida strains isolated from sheep pneumonia in Iran. Bacteriological and bio...

Antimicrobial Susceptibility of Pasteurella multocida Isolates

The sensitivity to antimicrobial agents of 27 Pasteurella multocida isolates was assayed in terms of minimum inhibitory concentration, employing a serial broth dilution technique. Prepared microtitre plates used in the study contained 17 drugs in four or eight dilutions, with four controls. Isolates of P. multocida were considered in four groups, depending on their origin (from field outbreaks;...